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h7sk grna expression cassette  (Addgene inc)


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    Structured Review

    Addgene inc h7sk grna expression cassette
    H7sk Grna Expression Cassette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h7sk+grna+expression+cassette/HA-EglN1-pcDNA3+(Plasmid+%2318963)/ppr0835066-380-9-16
    Average 93 stars, based on 15 article reviews
    h7sk grna expression cassette - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: High-throughput proteo-genomic identification of functional endothelial cell microproteins encoded by non-canonical small open reading frames
    Article Snippet: 707 Liquid chromatography and tandem mass spectrometry (LC-MS/MS) 708 LC-MS/MS analyzes where performed using an EASY-nLC 1200 nano flow chromatography 709 sytem interfacing with QExactive and QExactive HF mass spectrometers (all ThermoFisher 710 Scientific, Bremen, Germany) through electrospray ionization with in house packed column 711 emitters (20 cm, 75 μm ID, 1.9 μm C18 beads, Dr. Maisch GmbH, Ammerbuch, Germany) and 712 using a buffer system of 0.1% formic acid (solvent A) and 80% acetonitrile/0.1% formic acid 713 (solvent B). .. The oligos were then 767 specifically annealed to the h7SK gRNA expression cassette on 768 pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2 (Addgene #189632), analysed 769 by gel electrophoresis on a 0.8% agarose gel and the successful cloning of gRNA-containing 770 oligos into lentiviral plasmid was verified by SANGER sequencing. .. The quality and distribution 771 of the plasmid gRNA library was determined by NGS on an Illumina MiSeq sequencer using 772 a paired-end 2x74bp protocol, according to the manufacturer’s instructions.

    Nucleic Acid Electrophoresis:

    Article Title: High-throughput proteo-genomic identification of functional endothelial cell microproteins encoded by non-canonical small open reading frames
    Article Snippet: 707 Liquid chromatography and tandem mass spectrometry (LC-MS/MS) 708 LC-MS/MS analyzes where performed using an EASY-nLC 1200 nano flow chromatography 709 sytem interfacing with QExactive and QExactive HF mass spectrometers (all ThermoFisher 710 Scientific, Bremen, Germany) through electrospray ionization with in house packed column 711 emitters (20 cm, 75 μm ID, 1.9 μm C18 beads, Dr. Maisch GmbH, Ammerbuch, Germany) and 712 using a buffer system of 0.1% formic acid (solvent A) and 80% acetonitrile/0.1% formic acid 713 (solvent B). .. The oligos were then 767 specifically annealed to the h7SK gRNA expression cassette on 768 pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2 (Addgene #189632), analysed 769 by gel electrophoresis on a 0.8% agarose gel and the successful cloning of gRNA-containing 770 oligos into lentiviral plasmid was verified by SANGER sequencing. .. The quality and distribution 771 of the plasmid gRNA library was determined by NGS on an Illumina MiSeq sequencer using 772 a paired-end 2x74bp protocol, according to the manufacturer’s instructions.

    Agarose Gel Electrophoresis:

    Article Title: High-throughput proteo-genomic identification of functional endothelial cell microproteins encoded by non-canonical small open reading frames
    Article Snippet: 707 Liquid chromatography and tandem mass spectrometry (LC-MS/MS) 708 LC-MS/MS analyzes where performed using an EASY-nLC 1200 nano flow chromatography 709 sytem interfacing with QExactive and QExactive HF mass spectrometers (all ThermoFisher 710 Scientific, Bremen, Germany) through electrospray ionization with in house packed column 711 emitters (20 cm, 75 μm ID, 1.9 μm C18 beads, Dr. Maisch GmbH, Ammerbuch, Germany) and 712 using a buffer system of 0.1% formic acid (solvent A) and 80% acetonitrile/0.1% formic acid 713 (solvent B). .. The oligos were then 767 specifically annealed to the h7SK gRNA expression cassette on 768 pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2 (Addgene #189632), analysed 769 by gel electrophoresis on a 0.8% agarose gel and the successful cloning of gRNA-containing 770 oligos into lentiviral plasmid was verified by SANGER sequencing. .. The quality and distribution 771 of the plasmid gRNA library was determined by NGS on an Illumina MiSeq sequencer using 772 a paired-end 2x74bp protocol, according to the manufacturer’s instructions.

    Cloning:

    Article Title: High-throughput proteo-genomic identification of functional endothelial cell microproteins encoded by non-canonical small open reading frames
    Article Snippet: 707 Liquid chromatography and tandem mass spectrometry (LC-MS/MS) 708 LC-MS/MS analyzes where performed using an EASY-nLC 1200 nano flow chromatography 709 sytem interfacing with QExactive and QExactive HF mass spectrometers (all ThermoFisher 710 Scientific, Bremen, Germany) through electrospray ionization with in house packed column 711 emitters (20 cm, 75 μm ID, 1.9 μm C18 beads, Dr. Maisch GmbH, Ammerbuch, Germany) and 712 using a buffer system of 0.1% formic acid (solvent A) and 80% acetonitrile/0.1% formic acid 713 (solvent B). .. The oligos were then 767 specifically annealed to the h7SK gRNA expression cassette on 768 pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2 (Addgene #189632), analysed 769 by gel electrophoresis on a 0.8% agarose gel and the successful cloning of gRNA-containing 770 oligos into lentiviral plasmid was verified by SANGER sequencing. .. The quality and distribution 771 of the plasmid gRNA library was determined by NGS on an Illumina MiSeq sequencer using 772 a paired-end 2x74bp protocol, according to the manufacturer’s instructions.

    Plasmid Preparation:

    Article Title: High-throughput proteo-genomic identification of functional endothelial cell microproteins encoded by non-canonical small open reading frames
    Article Snippet: 707 Liquid chromatography and tandem mass spectrometry (LC-MS/MS) 708 LC-MS/MS analyzes where performed using an EASY-nLC 1200 nano flow chromatography 709 sytem interfacing with QExactive and QExactive HF mass spectrometers (all ThermoFisher 710 Scientific, Bremen, Germany) through electrospray ionization with in house packed column 711 emitters (20 cm, 75 μm ID, 1.9 μm C18 beads, Dr. Maisch GmbH, Ammerbuch, Germany) and 712 using a buffer system of 0.1% formic acid (solvent A) and 80% acetonitrile/0.1% formic acid 713 (solvent B). .. The oligos were then 767 specifically annealed to the h7SK gRNA expression cassette on 768 pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2 (Addgene #189632), analysed 769 by gel electrophoresis on a 0.8% agarose gel and the successful cloning of gRNA-containing 770 oligos into lentiviral plasmid was verified by SANGER sequencing. .. The quality and distribution 771 of the plasmid gRNA library was determined by NGS on an Illumina MiSeq sequencer using 772 a paired-end 2x74bp protocol, according to the manufacturer’s instructions.

    Sequencing:

    Article Title: High-throughput proteo-genomic identification of functional endothelial cell microproteins encoded by non-canonical small open reading frames
    Article Snippet: 707 Liquid chromatography and tandem mass spectrometry (LC-MS/MS) 708 LC-MS/MS analyzes where performed using an EASY-nLC 1200 nano flow chromatography 709 sytem interfacing with QExactive and QExactive HF mass spectrometers (all ThermoFisher 710 Scientific, Bremen, Germany) through electrospray ionization with in house packed column 711 emitters (20 cm, 75 μm ID, 1.9 μm C18 beads, Dr. Maisch GmbH, Ammerbuch, Germany) and 712 using a buffer system of 0.1% formic acid (solvent A) and 80% acetonitrile/0.1% formic acid 713 (solvent B). .. The oligos were then 767 specifically annealed to the h7SK gRNA expression cassette on 768 pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2 (Addgene #189632), analysed 769 by gel electrophoresis on a 0.8% agarose gel and the successful cloning of gRNA-containing 770 oligos into lentiviral plasmid was verified by SANGER sequencing. .. The quality and distribution 771 of the plasmid gRNA library was determined by NGS on an Illumina MiSeq sequencer using 772 a paired-end 2x74bp protocol, according to the manufacturer’s instructions.



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    Addgene inc h7sk grna expression cassette
    H7sk Grna Expression Cassette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h7sk+grna+expression+cassette/HA-EglN1-pcDNA3+(Plasmid+%2318963)/ppr0835066-380-9-16
    Average 93 stars, based on 1 article reviews
    h7sk grna expression cassette - by Bioz Stars, 2026-09
    93/100 stars
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